Individual Participant Data (IPD) Sharing Statement: Studies a U.S. FDA-regulated Drug Product: Studies a U.S. FDA-regulated Device Product: Change in the depressive symptoms severity [ Time Frame: 8 weeks ], Change in microbiota composition [ Time Frame: 8 weeks ], Changes in the serum levels of biomarkers [ Time Frame: 8 weeks ], Change in perception of stress [ Time Frame: 8 weeks ]. FEMS Immunol Med Microbiol, 66: 230-239 (4) Di Cerbo, Alessandro et al. Numerous products available have shown effectiveness. Isolates from BV/STI-negative women are shown by plain bars, those from BV and/or STI-positive women are patterned and ATCC strains are shown by black bars. (2016). Project administration, Ganz im Gegenteil kann das Milchsäurebakterium durch seine antimikrobiellen Eigenschaften sogar schädliche Keime aktiv bekämpfen und diese aus seinem Lebensraum verdrängen (1). In vivo, women with an L. crispatus-dominated vaginal microbiota have been shown to have a lower FGT pH than women with a L. gasseri- or L. jensenii-dominated microbiota [4], suggesting good alignment between these in vitro characteristics and in vivo phenotypes. MICs below the lowest or above the highest concentration tested were assigned a MIC half of the lowest or twice the highest concentration tested, respectively. While antibiotics are the standard-of-care for bacterial vaginosis, most cases recur within six-months. The Kruskal-Wallis one-way analysis of variance was used to compare variables with three or more groups. Perinatal HIV Research Unit (PHRU), Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa, Formal analysis, AUC for each growth curve was calculated and percentage of growth inhibition in the presence of Lactobacillus-conditioned MRS compared to the control calculated. The probiotic potential of these isolates was found to be strain-specific, and vaginal Lactobacillus strains largely performed better than probiotic strains currently used in probiotics for vaginal health, available internationally and in South Africa. Study record managers: refer to the Data Element Definitions if submitting registration or results information. All vaginal Lactobacillus isolates (n = 57) were resistant to metronidazole, while the susceptibility to clindamycin varied within and between Lactobacillus spp. Filled boxes indicate which strains were obtained from BV and/or STI-positive women. E-mail: Investigation, Cultures adjusted to OD600nm 0.1±0.01 in MRS broth were grown anaerobically at 37°C, and culture supernatant pH measured at 3, 6, 12, 24 and 48 hours using a calibrated pH meter. https://doi.org/10.1371/journal.ppat.1008559.g003. Konsequenzen dieses Ungleichgewichts können Funktionsstörungen des Verdauungstraktes, Störungen des Stoffwechsels und Neigung zu Übergewicht oder eine erhöhte Anfälligkeit für Magen-Darm-Infektionen sein. 30, 157-175. doi: 10.2500/ajra.2016.30.4354. (2012), Immunomodulating and anti-infective effects of a novel strain of Lactobacillus paracasei that strongly induces interleukin-12. Lactobacillus species was found in blood cultures of 15% of patients receiving ongoing treatment that included an antimicrobial agent to which the isolated bacteria was presumably susceptible (on the basis of E-test results). Draft de novo assemblies were prepared using a customised assembly pipeline. Verschiedene klinische Studien zeigten eine Besserung einer allergischen Rhinitis (Heuschnupfen) durch die regelmäßige Einnahme von probiotischen L. paracasei Stämmen. Few Lactobacillus strains were resistant to clindamycin, as described by others [56–58], indicating that these strains could not be administered concurrently with SOC clindamycin treatment. L.paracasei kann darüber hinaus sowohl im Labor in vitro als auch in vivo bei Mäusen pathogene, also krankheitserregende, Keime bekämpfen und diese verdrängen (8). The vaginal microbiota was characterised by 16s rRNA gene sequencing [36]. formally request permission using Copyright Clearance Center. The sequences have been deposited in the European Nucleotide Archive (ENA) under accession number PRJEB37955. BV can lead to severe reproductive complications [6–8], such as an increase in the risk for acquiring and transmitting sexually-transmitted infections (STIs) including human immunodeficiency virus (HIV) [9–11]. Here, we characterised a large panel of vaginal Lactobacillus strains from young African women (including growth, adhesion to host cells, ability to produce antimicrobial products, and pathogen inhibition), compared to isolates from commercial probiotic products for vaginal health. Vaginal probiotics may improve durability of BV treatment, although few probiotics for vaginal health contain Lactobacillus spp. For this ranking, a total of 36/57 Lactobacillus strains (for which complete characteristic evaluations were available) were included. Overall, L. crispatus strains tended to exhibit the strongest inhibitory activity against G. vaginalis, although this was not statistically significant. Trimmed reads were assembled using SPAdes v3.13.0 [71] using the ‘—careful’ flag and omitting the initial read error correction step. A total of 57 Lactobacillus strains were isolated from the FGT of 26 young (median age 18 years; IQR 17–19 years) South African women, including 10 L. crispatus, 9 L. gasseri, 18 L. jensenii, 8 L. vaginalis, and 12 L. mucosae strains (Table 1). Tel: +86-29-88460541, b do not need to formally request permission to reproduce material contained in this For a subset of Lactobacillus strains (n = 20), broader antibiotic susceptibility profiles were determined using Sensititre GPALL1F plates (including ampicillin, cefoxitin, chloramphenicol, ciprofloxacin, clindamycin, daptomycin, erythromycin, gentamicin, levofloxacin, linezolid, moxifloxacin, nitrofurantoin, oxacillin, penicillin, quinupristin/dalfopristin, rifampin, streptomycin, tetracycline, tigecycline, trimethoprim/sulamethoxazole and vancomycin; Thermo Fisher Scientific Inc., USA), according to the manufacturer’s instructions. Some vaginal strains produced high levels of L- but almost no D-lactate (such as L. crispatus 95.34PA), while others produced large amounts of D- but little L-lactate (such as L. jensenii 96.45PA). Information provided by (Responsible Party): Antonio L Teixeira Jr, Federal University of Minas Gerais. is available on our Permission Requests page. Double-layer disc diffusion was used to determine the susceptibility to metronidazole (5μg), clindamycin (2μg), penicillin (2μg) and amoxicillin (10μg; Davies Diagnostics, South Africa), as described previously [58]. It remains to be decided whether this probiotic should be region-specific, and only available to Southern African women due to the origin of its Lactobacillus strains, or worldwide. A wider range of well-characterized Lactobacillus-containing probiotics may improve bacterial vaginosis treatment outcomes, which would hopefully lower the burden of adverse pregnancy outcomes and sexually transmitted infections. Das Milchsäurebakterium kann die Barrierefunktion der Haut unterstützen, das Gleichgewicht der Haut aufrechterhalten und Beschwerden bei sensibler Haut verringern (12). (5) Feeding L. rhamnosus SHA113 caused a significant reduction of Proteobacteria in the cecum of healthy mice, which was not significant in infected and treated mice. Conditioned media from L. crispatus (pink), L. gasseri (blue), L. jensenii (green), L. vaginalis (orange), L. mucosae (grey) and probiotic (red, LR–L. Competing interests: The authors have declared that no competing interests exist. L. crispatus and L. jensenii strains grew the best, as quantified by calculation of the area under the curve (AUC), followed by L. vaginalis, L. mucosae and lastly L. gasseri strains. Earlier studies suggested that H2O2 was the most influential Lactobacillus spp.-produced metabolite to inhibit pathogens in the lower FGT, although these studies were conducted aerobically [39,40]. that commonly colonize the lower female genital tract. You do not have JavaScript enabled. Using a weighted PPP scoring system, the identified top-performing strains included only vaginal Lactobacillus strains, obtained both from BV/STI negative South African women as well as some from donors who had BV and/or STIs, while none of the commercially available strains ranked as well, suggesting that there is potential to improve currently available probiotic formulations for vaginal health.

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